{ "$schema": "https://json-schema.org/draft/2020-12/schema", "$id": "https://scischema.org/schemas/biology/electrical-cell-stimulation/1.0.0/master-schema.json", "version": "1.0.0", "title": "Electrical Cell Stimulation Process Schema", "description": "Schema representing key parameters for electrical cell stimulation experiments in tissue engineering and regenerative medicine.", "type": "object", "$defs": { "QuantityValue": { "type": "object", "description": "A reported quantity with value and unit.", "properties": { "value": { "type": "number", "description": "Numerical value of the quantity." }, "unit": { "type": "string", "description": "Measurement unit associated with the value." } }, "required": [ "value", "unit" ], "unevaluatedProperties": false } }, "properties": { "experiment_metadata": { "type": "object", "description": "Metadata about the experiment, including general information and timing.", "properties": { "experiment_name": { "type": "string", "description": "A descriptive name for the experiment." }, "experiment_description": { "type": "string", "description": "A short description of the experiment and which biological process is addressed." }, "experiment_date": { "type": "string", "format": "date", "description": "Date when the experiment was started (YYYY-MM-DD)." }, "experiment_time": { "type": "string", "format": "time", "description": "Time when the experiment was started (HH:MM:SS)." }, "experimenter": { "type": "string", "description": "Name or identifier of the experimenter." } }, "required": [ "experiment_date" ] }, "cell_culture": { "type": "object", "description": "Parameters related to cell preparation and culture conditions.", "properties": { "cell_type": { "type": "string", "description": "Name of the cell line or primary cell type used (e.g., 'MC3T3-E1', 'human mesenchymal stem cells').", "examples": [ "HEK293", "NIH/3T3", "primary osteoblasts" ] }, "cell_source": { "type": "string", "description": "Source of the cells (e.g., adipose tissue, bone marrow)." }, "passage_number": { "type": "integer", "minimum": 0, "description": "Passage number of the cells used in the experiment." }, "donor_information": { "type": "object", "description": "Information about the biological source of the cells, including donor age, sex, and tissue harvesting location (if applicable).", "properties": { "age": { "type": "number", "description": "Age of the donor in years.", "minimum": 0 }, "sex": { "type": "string", "enum": [ "male", "female", "unknown" ], "description": "Sex of the donor." }, "harvest_location": { "type": "string", "description": "Anatomical site from which tissue was harvested (e.g., abdominal, gluteal)." } }, "unevaluatedProperties": false }, "medium": { "type": "object", "description": "Composition of the cell culture medium including base medium and supplements.", "properties": { "base_medium": { "type": "string", "description": "Base culture medium formulation (e.g., 'DMEM', 'RPMI-1640').", "examples": [ "DMEM", "α-MEM", "EMEM" ] }, "ph_medium": { "type": "string", "description": "pH of complete cell culture medium." }, "supplements": { "type": "array", "items": { "type": "object", "properties": { "supplement_name": { "type": "string", "description": "Name of the supplement (e.g., FBS, penicillin-streptomycin)." }, "supplement_concentration": { "type": "string", "description": "Concentration of the supplement (e.g., 10% FBS, 1% Penicillin-Streptomycin, 50 μg/mL L-ascorbic acid)." }, "supplement_type": { "type": "string", "description": "Type of supplement (e.g., 'growth factor', 'antibiotic')." } }, "required": [ "supplement_name", "supplement_concentration", "supplement_type" ] }, "description": "List of supplements used in the cell culture medium." } }, "required": [ "base_medium" ], "unevaluatedProperties": false }, "seeding_density": { "$ref": "#/$defs/QuantityValue", "description": "Initial cell density at time of plating (critical for confluency and stimulation response).", "properties": { "value": { "minimum": 0 }, "unit": { "enum": [ "cells/cm^2", "cells/mL" ] } } }, "cell_plating": { "type": "object", "description": "Details of cell plating procedure.", "properties": { "array_support_type": { "type": "string", "description": "Reaction and culture vessels that are used during the experiment (e.g., '6-well plate', 'single flask')." }, "surface_coating": { "type": "string", "description": "Type of surface coating or substrate used (e.g., 'Titanium', 'Poly-L-lysine')." }, "adhesion_time": { "$ref": "#/$defs/QuantityValue", "description": "Time allowed for cells to adhere to the surface.", "properties": { "value": { "minimum": 0 }, "unit": { "enum": [ "s", "min", "h" ] } } } } }, "additional_components": { "type": "object", "description": "Details of additional scaffolds or substrates used for cell plating procedure (if applicable).", "properties": { "scaffold_type": { "type": "string", "description": "Type of scaffold/substrate used (e.g., '3D bioprinted scaffold', 'TiO2 nanotubes', 'polycaprolactone')." } } }, "incubator_environment": { "type": "object", "description": "Environmental conditions for cell culture.", "properties": { "gas_phase_composition": { "type": "array", "description": "Composition of gases in the incubator atmosphere, including gas type and concentration.", "items": { "type": "object", "properties": { "gas": { "type": "string", "description": "Type of gas (e.g., O2, CO2)." }, "percentage": { "type": "number", "minimum": 0, "maximum": 100, "description": "Percentage of gas in the mixture." } }, "required": [ "gas", "percentage" ] } }, "temperature": { "$ref": "#/$defs/QuantityValue", "description": "Temperature in °C.", "properties": { "unit": { "const": "°C" } } }, "humidity": { "type": "number", "minimum": 0, "maximum": 100, "description": "Relative humidity in percent." } } } }, "required": [ "cell_type", "medium", "seeding_density" ], "unevaluatedProperties": false }, "electrical_stimulation": { "type": "object", "description": "Parameters defining the electrical stimulation configuration.", "properties": { "stimulation_chamber": { "type": "object", "description": "Features of the electrical stimulation chamber.", "properties": { "stimulation_device": { "type": "string", "description": "Device used for electrical stimulation (e.g., 'function generator', 'IsoStim', 'amplifier')." }, "electrode_material": { "type": "string", "description": "Conductive material used for electrodes.", "enum": [ "carbon", "gold", "iridium", "indium tin oxide", "platinum", "salt bridges", "titanium", "other" ] }, "surface_roughness": { "$ref": "#/$defs/QuantityValue", "description": "Surface roughness of the electrode in micrometers (μm).", "properties": { "unit": { "const": "μm" } } }, "electrode_spacing": { "$ref": "#/$defs/QuantityValue", "description": "Distance between the electrodes in millimeters (mm).", "properties": { "unit": { "const": "mm" } } }, "electrode_configuration": { "type": "string", "description": "Configuration of electrodes in the chamber (e.g., L-shaped rods, parallel plates)." }, "cell_location": { "type": "string", "description": "Location of cells relative to electrodes.", "enum": [ "on electrode", "on coverslip between electrodes", "on substrate between electrodes", "other" ] } } }, "stimulation_parameters": { "type": "object", "description": "Electrical signal characteristics applied during stimulation.", "properties": { "stimulation_type": { "type": "string", "description": "Type of electrical stimulation (e.g., 'capacitive', 'direct (contact)', 'inductive').", "enum": [ "direct (contact)", "capacitive", "inductive", "other" ] }, "frequency": { "$ref": "#/$defs/QuantityValue", "description": "Stimulation frequency in hertz (Hz). Must be >0 for AC stimulation, 0 for DC stimulation.", "properties": { "value": { "minimum": 0 }, "unit": { "const": "Hz" } } }, "waveform": { "type": "string", "enum": [ "DC", "sinusoidal", "biphasic rectangular", "monophasic rectangular", "other" ], "description": "Shape of the electrical waveform applied during stimulation." }, "duty_cycle": { "type": "number", "minimum": 0, "maximum": 100, "description": "Duty cycle in percent (for rectangular waves only)." }, "charge_balanced": { "type": "boolean", "description": "Indicates if the stimulation waveform is charge-balanced to prevent net charge transfer." }, "electrical_stimulation_duration": { "type": "object", "description": "The total duration of the electrical stimulation experiment.", "properties": { "total_duration_value": { "type": "number", "description": "Numerical duration value.", "minimum": 0 }, "total_duration_unit": { "type": "string", "description": "Time unit (seconds, minutes, hours, or days).", "enum": [ "s", "min", "h", "d" ] }, "stimulation_cycles": { "type": "object", "description": "Parameters for intermittent stimulation cycles.", "properties": { "cycles_per_day": { "type": "integer", "description": "Number of stimulation cycles per day.", "minimum": 0 }, "cycle_duration": { "$ref": "#/$defs/QuantityValue", "description": "Duration of each stimulation cycle.", "properties": { "value": { "minimum": 0 }, "unit": { "enum": [ "s", "min", "h" ] } } }, "interval_between_cycles": { "$ref": "#/$defs/QuantityValue", "description": "Time interval between stimulation cycles.", "properties": { "value": { "minimum": 0 }, "unit": { "enum": [ "s", "min", "h" ] } } } }, "required": [ "cycles_per_day", "cycle_duration", "interval_between_cycles" ], "unevaluatedProperties": false } }, "required": [ "total_duration_value", "total_duration_unit" ], "unevaluatedProperties": false }, "applied_signal_strength": { "type": "object", "description": "Applied electrical signal strength.", "properties": { "applied_signal": { "type": "string", "description": "Type of electrical signal applied during stimulation, such as voltage or current.", "enum": [ "Voltage", "Current" ] }, "applied_signal_value": { "type": "number", "description": "Numerical value for the applied electrical signal.", "minimum": 0 }, "applied_signal_unit": { "type": "string", "description": "Unit of the applied electrical signal, such as V or A.", "default": "V" }, "resulting_current_density_value": { "type": "number", "description": "Calculated current density at cells.", "minimum": 0 }, "resulting_current_density_unit": { "type": "string", "description": "Unit of the resulting current density, such as mA/cm².", "default": "mA/cm^2" } }, "required": [ "applied_signal", "applied_signal_value", "applied_signal_unit" ], "allOf": [ { "if": { "properties": { "applied_signal": { "const": "Voltage" } } }, "then": { "properties": { "applied_signal_unit": { "enum": [ "mV", "V" ], "default": "V" } } } }, { "if": { "properties": { "applied_signal": { "const": "Current" } } }, "then": { "properties": { "applied_signal_unit": { "enum": [ "μA", "mA" ] } } } } ], "unevaluatedProperties": false } }, "allOf": [ { "if": { "properties": { "waveform": { "enum": [ "monophasic rectangular", "biphasic rectangular" ] } } }, "then": { "required": [ "duty_cycle" ] } } ], "required": [ "frequency", "waveform", "electrical_stimulation_duration", "applied_signal_strength" ], "unevaluatedProperties": false }, "field_strength": { "type": "object", "properties": { "field_strength_value": { "type": "number", "description": "Numerical electric field strength value (measured, simulated, or calculated from applied voltage and electrode spacing).", "minimum": 0 }, "field_strength_unit": { "type": "string", "description": "Electric field strength unit.", "enum": [ "V/m (same as mV/mm)", "V/cm" ] }, "determined_by": { "type": "string", "description": "Source of E-field determination.", "enum": [ "measurements", "simulations", "calculations" ] } }, "required": [ "field_strength_value", "field_strength_unit" ], "description": "The electric field strength (E-field) is a key parameter in electic stimulation studies and is essential for reproducibility and comparison across experiments." }, "field_simulation": { "type": "object", "description": "Details of electric field simulation and validation (if applicable).", "properties": { "simulation_method": { "type": "string", "description": "Method used for electric field simulation (e.g., 'FEM', 'analytical calculations').", "examples": [ "Finite Element Analysis", "COMSOL Multiphysics", "SolidWorks CAD" ] }, "software": { "type": "string", "description": "Simulation software used." }, "software_version": { "type": "string", "description": "Version of simulation software (e.g., 0.3)." }, "mesh_size": { "type": "number", "description": "Mesh size used in simulation.", "minimum": 0 }, "mesh_unit": { "type": "string", "description": "Unit for mesh size.", "enum": [ "mm", "cm", "m" ] }, "validation_method": { "type": "string", "description": "Method used to validate electric field distribution." }, "homogeneous_field": { "type": "string", "enum": [ "homogeneous", "non-homogeneous" ], "description": "Homogeneity of the electric field distribution." } }, "required": [ "simulation_method", "validation_method" ], "unevaluatedProperties": false } }, "required": [ "stimulation_chamber", "stimulation_parameters" ], "unevaluatedProperties": false }, "experimental_procedure": { "type": "array", "description": "Step-by-step description of the experimental workflow.", "items": { "type": "object", "properties": { "action": { "type": "string", "description": "Primary action performed in this step.", "enum": [ "add", "block", "fix", "harvest", "image", "incubate", "lyse", "measure", "mix", "observe", "other", "permeabilize", "seed", "stain", "stimulate", "wash" ] }, "details": { "type": "string", "description": "Additional parameters and specifications for the step (e.g., 'with PBS for 5 min at 37°C')." } }, "required": [ "action" ], "unevaluatedProperties": false } }, "experimental_analysis": { "type": "object", "description": "Methods and materials used for post-stimulation evaluation.", "properties": { "alkaline_phosphatase": { "type": "object", "description": "Analysis of alkaline phosphatase (ALP) activity, including method, time points, group values, and unit.", "properties": { "alkaline_phosphatase_method": { "type": "string", "description": "Method used for alkaline phosphatase analyses." }, "alkaline_phosphatase_results": { "type": "array", "description": "A list of results from the alkaline phosphatase analyses at different time points.", "items": { "type": "object", "properties": { "alkaline_phosphatase_time_value": { "type": "number", "description": "Time points after start of electrical stimulation at which alkaline phosphatase was analyzed.", "minimum": 0 }, "alkaline_phosphatase_time_unit": { "type": "string", "description": "The unit of time in minutes, hours, or days.", "enum": [ "min", "h", "d" ] }, "alkaline_phosphatase_result_value_ES": { "type": "number", "minimum": 0, "description": "Alkaline phosphatase activity level of stimulated group." }, "alkaline_phosphatase_result_value_control": { "type": "number", "minimum": 0, "description": "Alkaline phosphatase activity level of control." }, "alkaline_phosphatase_result_unit": { "type": "string", "description": "Unit of measurement for alkaline phosphatase activity." } } } } } }, "apoptosis_assessment": { "type": "object", "description": "Assessment of apoptosis after electrical stimulation, including method, time points, and group values.", "properties": { "apoptosis_analysis_method": { "type": "string", "description": "Method used for apoptosis assessment (e.g., Annexin V staining, TUNEL assay)." }, "apoptosis_results": { "type": "array", "description": "A list of results from the apoptosis analyses at different time points.", "items": { "type": "object", "properties": { "apoptosis_analysis_time_value": { "type": "number", "description": "Time points after start of electrical stimulation at which apoptosis analysis was performed.", "minimum": 0 }, "apoptosis_analysis_time_unit": { "type": "string", "description": "The unit of time in minutes, hours, or days.", "enum": [ "min", "h", "d" ] }, "apoptosis_result_ES": { "type": "number", "minimum": 0, "description": "Percentage of apoptotic cells of stimulated group." }, "apoptosis_result_control": { "type": "number", "minimum": 0, "description": "Percentage of apoptotic cells of control." } } } } } }, "calcium_ion_signaling": { "type": "object", "description": "Analysis of intracellular calcium ion signaling.", "properties": { "calcium_ion_signaling_method": { "type": "string", "description": "Method used for calcium ion detection." }, "calcium_ion_signaling_results": { "type": "array", "description": "A list of results from the calcium ion detection at different time points.", "items": { "type": "object", "properties": { "calcium_ion_signaling_time_value": { "type": "number", "description": "Time points after start of electrical stimulation at which calcium signals were analyzed.", "minimum": 0 }, "calcium_ion_signaling_time_unit": { "type": "string", "description": "The unit of time in minutes, hours, or days.", "enum": [ "min", "h", "d" ] }, "calcium_ion_signaling_ES": { "type": "number", "minimum": 0, "description": "Calcium signal stimulated group." }, "calcium_ion_signaling_control": { "type": "number", "minimum": 0, "description": "Calcium signal control." } } } } } }, "calcium_deposition": { "type": "object", "description": "Analysis of calcium deposition or mineralization, including method, time points, group values, and unit.", "properties": { "calcium_deposition_method": { "type": "string", "description": "Method used for calcium deposition analyses." }, "calcium_deposition_results": { "type": "array", "description": "A list of results from the calcium deposition analyses at different time points.", "items": { "type": "object", "properties": { "calcium_deposition_time_value": { "type": "number", "description": "Time points after start of electrical stimulation at which calcium deposition was analyzed.", "minimum": 0 }, "calcium_deposition_time_unit": { "type": "string", "description": "The unit of time in minutes, hours, or days.", "enum": [ "min", "h", "d" ] }, "calcium_deposition_result_value_ES": { "type": "number", "minimum": 0, "description": "Calcium deposition level of stimulated group." }, "calcium_deposition_result_value_control": { "type": "number", "minimum": 0, "description": "Calcium deposition level of control." }, "calcium_deposition_result_unit": { "type": "string", "description": "Unit of measurement for calcium deposition." } } } } } }, "cell_cycle_analysis": { "type": "object", "description": "Analysis of cell-cycle distribution, including method, time points, group values, and phase-related results.", "properties": { "method": { "type": "string", "description": "Method used for cell cycle analysis (e.g., EdU assay, flow cytometry)." }, "cell_cycle_results": { "type": "array", "description": "A list of results from the cell cycle analyses at different time points.", "items": { "type": "object", "properties": { "cell_cycle_analysis_time_value": { "type": "number", "description": "Time points after start of electrical stimulation at which cell cycle analysis was performed.", "minimum": 0 }, "cell_cycle_analysis_time_unit": { "type": "string", "description": "The unit of time in minutes, hours, or days.", "enum": [ "min", "h", "d" ] }, "G0_G1": { "type": "number", "description": "Percentage of cells in G0/G1 phase." }, "S": { "type": "number", "description": "Percentage of cells in S phase." }, "G2_M": { "type": "number", "description": "Percentage of cells in G2/M phase." }, "sub_G1": { "type": "number", "description": "Percentage of cells in sub-G1 phase (indicating apoptosis)." } } } } } }, "cytoskeleton_orientation": { "type": "object", "description": "Analysis of cytoskeleton orientation relative to the electric field.", "properties": { "cytoskeleton_orientation_measurement_method": { "type": "string", "description": "Method used for measuring cytoskeleton orientation." }, "cytoskeleton_orientation_angle": { "type": "number", "description": "Angle of cytoskeleton orientation in degrees." }, "cytoskeleton_orientation_percentage": { "type": "number", "description": "Percentage of cells with cytoskeleton aligned perpendicular to the electric field." } } }, "electrical_impedance_spectroscopy": { "type": "object", "description": "Impedance measurements taken during the experiment.", "properties": { "method": { "type": "string", "description": "Method used for impedance measurement (e.g., three-point method, four-point method)." }, "start_frequency": { "type": "number", "description": "Start frequency in Hertz (Hz)." }, "end_frequency": { "type": "number", "description": "End frequency in Hertz (Hz)." } }, "required": [ "start_frequency", "end_frequency" ] }, "hydrogen_peroxide": { "type": "object", "description": "Hydrogen peroxide (H2O2) concentration measurements.", "properties": { "hydrogen_peroxide_method": { "type": "string", "description": "The method used for the H2O2 measurements." }, "hydrogen_peroxide_measurement_results": { "type": "array", "description": "A list of results from the H2O2 measurements at different time points.", "items": { "type": "object", "properties": { "hydrogen_peroxide_time_value": { "type": "number", "description": "Time point after start of electrical stimulation at which H2O2 was measured.", "minimum": 0 }, "hydrogen_peroxide_time_unit": { "type": "string", "description": "The unit of time in minutes, hours, or days.", "enum": [ "min", "h", "d" ] }, "hydrogen_peroxide_concentration_ES": { "type": "number", "description": "The H2O2 concentration of ES group.", "minimum": 0 }, "hydrogen_peroxide_concentration_control": { "type": "number", "description": "The H2O2 concentration of control.", "minimum": 0 }, "hydrogen_peroxide_unit": { "type": "string", "description": "The unit of the H2O2 concentration.", "enum": [ "μM", "mM" ] } }, "required": [ "hydrogen_peroxide_time_value", "hydrogen_peroxide_time_unit", "hydrogen_peroxide_concentration_ES", "hydrogen_peroxide_concentration_control" ] } } } }, "imaging": { "type": "object", "description": "Imaging-specific parameters (required only if imaging is in investigation_methods).", "properties": { "imaging_device": { "type": "string", "enum": [ "confocal microscope", "electron microscope", "fluorescence microscope", "light microscope", "other microscope", "phase-contrast microscope", "two-photon microscope" ], "description": "Type of imaging device used." }, "resolution": { "type": "object", "properties": { "spatial_x": { "type": "number", "description": "Spatial resolution (x direction) in micrometers (μm).", "minimum": 0 }, "spatial_y": { "type": "number", "description": "Spatial resolution (y direction) in micrometers (μm).", "minimum": 0 }, "spatial_z": { "type": "number", "description": "Spatial resolution (z direction) in micrometers (μm).", "minimum": 0 }, "temporal": { "type": "number", "description": "Temporal resolution in seconds or milliseconds (e.g., frame rate).", "minimum": 0 } }, "description": "Resolution characteristics of the imaging device." }, "magnification": { "type": "number", "description": "Magnification level used (e.g., 10x, 20x, 40x, 60x). Write number only.", "minimum": 0 }, "primary_antibodies": { "type": "array", "description": "List of primary antibodies used for specific protein detection in fluorescence imaging.", "items": { "type": "object", "properties": { "name": { "type": "string", "description": "Name of the antibody." }, "dilution_factor": { "type": "string", "description": "Dilution factor (e.g., 1:100)." } }, "required": [ "name", "dilution_factor" ] } } }, "unevaluatedProperties": false }, "nuclei_aspect_ratio": { "type": "object", "description": "Analysis of nuclei aspect ratio indicating cell elongation.", "properties": { "nuclei_aspect_ratio_measurement_method": { "type": "string", "description": "Method used for measuring nuclei aspect ratio." }, "nuclei_aspect_ratio_result": { "type": "string", "description": "Individual measurements of nuclei aspect ratio." } } }, "pH_level": { "type": "object", "description": "pH level measurements during the experiment.", "properties": { "pH_level_method": { "type": "string", "description": "The method used for the pH measurements." }, "pH_level_measurement_results": { "type": "array", "description": "A list of results from the viability assay at different time points.", "items": { "type": "object", "properties": { "pH_level_time_value": { "type": "number", "description": "Time point after start of electrical stimulation at which pH was measured.", "minimum": 0 }, "pH_level_time_unit": { "type": "string", "description": "The unit of time in minutes, hours, or days.", "enum": [ "min", "h", "d" ] }, "pH_level_value_ES": { "type": "number", "description": "The pH value of stimulated group.", "minimum": 0, "maximum": 14 }, "pH_level_value_control": { "type": "number", "description": "The pH value of control.", "minimum": 0, "maximum": 14 } }, "required": [ "pH_level_time_value", "pH_level_time_unit", "pH_level_value_ES", "pH_level_value_control" ] } } } }, "proliferation": { "type": "object", "description": "Cell proliferation data at different time points.", "properties": { "cell_number_results": { "type": "array", "description": "A list of results from the cell number measurement at different time points.", "items": { "type": "object", "properties": { "cell_number_time_value": { "type": "number", "description": "Time points at which the cell number was analyzed.", "minimum": 0 }, "cell_number_time_unit": { "type": "string", "description": "The unit of time in minutes, hours, or days.", "enum": [ "min", "h", "d" ] }, "cell_number_ES": { "type": "number", "minimum": 0, "description": "Number of cells in stimulated samples." }, "cell_number_control": { "type": "number", "minimum": 0, "description": "Number of cells in controls." } } } }, "surface_coverage_results": { "type": "array", "description": "A list of results from the surface coverage measurement at different time points.", "items": { "type": "object", "properties": { "surface_coverage_time_value": { "type": "number", "description": "Time points after start of electrical stimulation at which the surface coverage was analyzed.", "minimum": 0 }, "surface_coverage_time_unit": { "type": "string", "description": "The unit of time in minutes, hours, or days.", "enum": [ "min", "h", "d" ] }, "surface_coverage_ES": { "type": "number", "minimum": 0, "description": "Percentage of surface coverage in stimulated samples." }, "surface_coverage_control": { "type": "number", "minimum": 0, "description": "Percentage of surface coverage in controls." } } } } } }, "reactive_oxygen_species": { "type": "object", "description": "Analysis of reactive oxygen species (ROS) production.", "properties": { "ROS_method": { "type": "string", "description": "Method used for ROS detection." }, "ROS_results": { "type": "array", "description": "A list of results from the ROS measurements at different time points.", "items": { "type": "object", "properties": { "ROS_measurement_time_value": { "type": "number", "description": "Time points after start of electrical stimulation at which ROS was analyzed.", "minimum": 0 }, "ROS_measurement_time_unit": { "type": "string", "description": "The unit of time in minutes, hours, or days.", "enum": [ "min", "h", "d" ] }, "ROS_value_ES": { "type": "number", "minimum": 0, "description": "ROS value of stimulated group." }, "ROS_value_control": { "type": "number", "minimum": 0, "description": "ROS value of control." }, "ROS_unit": { "type": "string", "description": "Unit of ROS measurement." } } } } } }, "viability_assay": { "type": "object", "description": "Results from the viability assay, typically using alamarBlue or similar methods.", "properties": { "viability_assay_method": { "type": "string", "description": "The method used for the viability assay (e.g., 'alamarBlue', 'MTT')." }, "viability_assay_results": { "type": "array", "description": "A list of results from the viability assay at different time points.", "items": { "type": "object", "properties": { "viability_assay_time_value": { "type": "number", "description": "The time point after begin of the stimulation experiment at which the viability assay was conducted.", "minimum": 0 }, "viability_assay_time_unit": { "type": "string", "description": "The unit of measurement.", "enum": [ "min", "h", "d" ] }, "viability": { "type": "number", "description": "The viability percentage relative to the control at the given time point.", "minimum": 0, "maximum": 100 } } } } }, "required": [ "viability_assay_method", "viability_assay_results" ] }, "additional_quantitative_data": { "type": "array", "items": { "type": "object", "properties": { "assay_type": { "type": "string", "enum": [ "AlamarBlue assay", "Alizarin Red staining", "ELISA", "flow cytometry", "gene expression profiling", "immunofluorescence", "live/dead staining", "MTT assay", "opg_expression", "osteocalcin", "other", "PCR", "qPCR", "rankl_expression", "runx2", "RT-PCR", "Western blot" ], "description": "List of analytical techniques used to assess stimulation effects." }, "metric": { "type": "string", "description": "Name of the metric being measured (e.g., cell proliferation rate, protein expression, ALP activity)." }, "quantitative_data_value_ES": { "type": "number", "description": "Measured value of the metric of stimulated group." }, "quantitative_data_value_control": { "type": "number", "description": "Measured value of the metric of control." }, "quantitative_data_unit": { "type": "string", "description": "Unit of measurement (e.g., %, intensity, number of cells)." }, "statistical_significance": { "type": "string", "description": "Statistical significance of the result (e.g., 'p < 0.05', 'p < 0.01')." } }, "required": [ "metric", "quantitative_data_value_ES", "quantitative_data_value_control", "quantitative_data_unit" ] }, "description": "Additional quantitative data related to the experiment outcomes." } }, "required": [], "unevaluatedProperties": false } }, "required": [ "cell_culture", "electrical_stimulation", "experimental_procedure", "experimental_analysis" ], "unevaluatedProperties": false }